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Modulation of neurotransmission is key for organismal responses to varying physiological contexts such as during infection, injury, or other stresses, as well as in learning and memory and for sensory adaptation. Roles for cell autonomous neuromodulatory mechanisms in these processes have been well described. The importance of cell non-autonomous pathways for inter-tissue signaling, such as gut-to-brain or glia-to-neuron, has emerged more recently, but the cellular mechanisms mediating such regulation remain comparatively unexplored. Glycoproteins and their G protein-coupled receptors (GPCRs) are well-established orchestrators of multi-tissue signaling events that govern diverse physiological processes through both cell-autonomous and cell non-autonomous regulation. Here, we show that follicle stimulating hormone receptor, FSHR-1, the soleCaenorhabditis elegansortholog of mammalian glycoprotein hormone GPCRs, is important for cell non-autonomous modulation of synaptic transmission. Inhibition offshr-1expression reduces muscle contraction and leads to synaptic vesicle accumulation in cholinergic motor neurons. The neuromuscular and locomotor defects infshr-1loss-of-function mutants are associated with an underlying accumulation of synaptic vesicles, build-up of the synaptic vesicle priming factor UNC-10/RIM, and decreased synaptic vesicle release from cholinergic motor neurons. Restoration of FSHR-1 to the intestine is sufficient to restore neuromuscular activity and synaptic vesicle localization tofshr-1-deficient animals. Intestine-specific knockdown of FSHR-1 reduces neuromuscular function, indicating FSHR-1 is both necessary and sufficient in the intestine for its neuromuscular effects. Re-expression of FSHR-1 in other sites of endogenous expression, including glial cells and neurons, also restored some neuromuscular deficits, indicating potential cross-tissue regulation from these tissues as well. Genetic interaction studies provide evidence that downstream effectorsgsa-1/Gα_S,acy-1/adenylyl cyclase andsphk-1/sphingosine kinase and glycoprotein hormone subunit orthologs, GPLA-1/GPA2 and GPLB-1/GPB5, are important for intestinal FSHR-1 modulation of the NMJ. Together, our results demonstrate that FSHR-1 modulation directs inter-tissue signaling systems, which promote synaptic vesicle release at neuromuscular synapses. 

Our research aims to highlight and alleviate the complex tensions around online safety, privacy, and smartphone usage in families so that parents and teens can work together to better manage mobile privacy and security-related risks. We developed a mobile application ("app") for Community Oversight of Privacy and Security ("CO-oPS") and had parents and teens assess whether it would be applicable for use with their families. CO-oPS is an Android app that allows a group of users to co-monitor the apps installed on one another's devices and the privacy permissions granted to those apps. We conducted a study with 19 parent-teen (ages 13-17) pairs to understand how they currently managed mobile safety and app privacy within their family and then had them install, use, and evaluate the CO-oPS app. We found that both parents and teens gave little consideration to online safety and privacy before installing new apps or granting privacy permissions. When using CO-oPS, participants liked how the app increased transparency into one another's devices in a way that facilitated communication, but were less inclined to use features for in-app messaging or to hide apps from one another. Key themes related to power imbalances between parents and teens surfaced that made co-management challenging. Parents were more open to collaborative oversight than teens, who felt that it was not their place to monitor their parents, even though both often believed parents lacked the technological expertise to monitor themselves. Our study sheds light on why collaborative practices for managing online safety and privacy within families may be beneficial but also quite difficult to implement in practice. We provide recommendations for overcoming these challenges based on the insights gained from our study. 

Managing digital privacy and security is often a collaborative process, where groups of individuals work together to share information and give one another advice. Yet, this collaborative process is not always reciprocal or equally shared. In many cases, individuals with more expertise help others without receiving help in return. Therefore, we studied the phenomenon of "Tech Caregiving" by surveying 20 groups (112 individuals) comprised of friends, family members, and/or co-workers who identified at least one member of their group as a someone who provides informal technical support to the people they know. We found that tech caregivers reported significantly higher levels of power use and self-efficacy for digital privacy and security, compared to tech caregivees. However, caregivers and caregivees did not differ based on their self-reportedcommunity collective-efficacy for collaboratively managing privacy and security together as a group. This finding demonstrates the importance of tech caregiving and community belonging in building community collective efficacy for digital privacy and security. We also found that caregivers and caregivees most often communicated via text message or phone when coordinating support, which was most frequently needed when troubleshooting or setting up new devices. Meanwhile, discussions specific to privacy and security represented only a small fraction of the issues for which participants gave or received tech care. Thus, we conclude that educating tech caregivers on how to provide privacy and security-focused support, as well as designing technologies that facilitate such support, has the potential to create positive networks effects towards the collective management of digital privacy and security.